Analysis of Chlorophyll Content and Photosynthetic Rate of Spring Maize by Chlorophyll Analyzer

The content of chlorophyll has a very important influence on the physiological activity of leaves and is one of its important indicators. This has a very close relationship with the ability of photosynthesis of leaves. Therefore, the determination of chlorophyll content can be used as a theory to increase crop yield. basis. The related laws of chlorophyll composition and content in summer maize leaves have been studied. Based on this, the changes of chlorophyll content in spring maize were systematically studied, and the data were used to increase the chlorophyll content of the leaves and accelerate the process of photosynthesis. For the determination of leaf chlorophyll content, chlorophyll analyzer can be used for effective analysis and measurement, which is convenient for research.

The changes of chlorophyll content at different leaf ages on the 9th, 15th and 21st leaves of spring maize under different nutrient conditions were determined by chlorophyll analyzer. The results showed that the chlorophyll content of the leaves was a single-peak curve change, and the peak appeared at 10-15 days after the leaf was unfolded. The peak of photosynthetic rate is delayed about one week. The photosynthetic rate of single leaf increased with the increase of chlorophyll content and decreased with the decrease of chlorophyll content. However, after the peaks of the two curves continued, the rate of decline of photosynthetic rate was faster than the rate of decline of chlorophyll content, and it was more pronounced with leaf senescence. Chlorophyll is present in the chloroplast, an important photosynthesis organ, and its content affects the absorption and conversion of light energy. With the aging of the leaves and the aging of the tissues, their ability to capture light energy and convert them into chemical energy is reduced, and the photosynthetic rate is significantly reduced. Therefore, although the chlorophyll content of young leaves and senescent leaves are comparable, the photosynthetic rate of the old leaves is significantly reduced. Without fertilization, leaf nitrogen deficiency is severe, accelerating the senescence of leaf tissue, but also accelerate the decomposition of proteins, enzymes and nitrogen transfer. After nitrogen application, the chlorophyll content and photosynthetic rate in the later period were significantly increased. Nitrogen, phosphorus and potassium, nitrogen, phosphorus and potassium treatments further improved the status of nitrogen, phosphorus and potassium in leaves, promoted the synthesis of protein, nucleic acid, chlorophyll, enzymes and ATP in leaves, and later increased chlorophyll content and photosynthetic rate.

Chlorophyll analyzer can determine the chlorophyll content of different leaf positions. The change is determined by the internal structure. The ultrastructure of the chloroplast in the middle leaves is the most complex. The photosynthetic membrane is especially developed, and there are more vascular bundles in the leaves and the vascular bundles. Chloroplasts in cells have a high degree of starch granules and therefore have a high chlorophyll content under the same conditions. Nitrogen is an essential component of chlorophyll. The lack of nitrogen in the non-fertilizer treatment affects the synthesis of chlorophyll. In the absence of nitrogen, the protein is decomposed and the water-soluble nitrogen compounds are transferred to the site of vigorous growth, accelerating the decomposition of chlorophyll in the lower leaves. The leaves are yellow. Chlorophyll molecules play a role in the absorption and transmission of light energy in photosynthesis, and the chlorophyll content increases, which increases the efficiency of light energy conversion. Nitrogen application significantly increased the chlorophyll content and photosynthetic rate of the lower leaves. Phosphorus not only interacts with nitrogen, but also promotes the synthesis of chlorophyll. This is the main reason for the further increase of chlorophyll content and photosynthetic rate of nitrogen, phosphorus and potassium.

Through the chlorophyll analyzer's detailed analysis of the chlorophyll content of spring maize leaves, it can be concluded that the use of chlorophyll content to express the photosynthetic rate of different leaf ages is relatively low. Because photosynthesis in photosynthesis is carried out in a certain membrane structure and a certain molecular arrangement. On the one hand, the old leaves of the basal thylakoid membrane began to loosen. On the other hand, the transfer of N from the leaf to the new leaves resulted in decomposition of a certain amount of pigment proteins and enzymes on the photosynthetic membrane, and even affected the molecular arrangement. Measures are taken in cultivation to regulate the structure and function of the photosynthetic membrane, so as to facilitate the assimilation of more photosynthetic products.

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